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Promega oligo d(t)15 primer
Oligo D(t)15 Primer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/oligo+d(t)15/oligo+dt++primers/pm38507920-86-27-30
Average 90 stars, based on 1 article reviews
oligo d(t)15 primer - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Reverse Transcription:

Article Title: Aluminum (Al) causes a delayed suppression of nucleotide excision repair (NER) capacity in zebrafish (Danio rerio) embryos via disturbance of DNA lesion detection.
Article Snippet: Aluminum (Al) is extensively used for making cooking utensils and its presence in the aquatic environment may occur through acid mine drainage and wastewater discharge.. Al is known to induce genotoxicity in human cells, rodents, and fish.. Nucleotide excision repair (NER) eliminates helix-twisting DNA lesions such as UV-induced dipyrimidine photoproducts.

Article Title: Increased expression of CD38 on endothelial cells in SARS-CoV-2 infection in cynomolgus macaques.
Article Snippet: SARS-CoV-2 infection causes activation of endothelial cells (ECs), leading to dysmorphology and dysfunction.. To study the pathogenesis of endotheliopathy, the activation of ECs in lungs of cynomolgus macaques after SARSCoV-2 infection and changes in nicotinamide adenine dinucleotide (NAD) metabolism in ECs were investigated, with a focus on the CD38 molecule, which degrades NAD in inflammatory responses after SARS-CoV-2 infection.. Activation of ECs was seen from day 3 after SARS-CoV-2 infection in macaques, with increases of intravascular fibrin and NAD metabolism-associated enzymes including CD38.

Article Title: Effects of salinity changes on the osmoregulatory and stress responses in the bay scallop Argopecten irradians
Article Snippet: Most marine mollusks exhibit osmoregulatory mechanisms to cope with hypoor hyperosmotic stress.. In the present study, we elucidated the effects of exposure to hyposmotic and hyperosmotic stress on bay scallop Argopecten irradians.. The Na+, K+, and Cl− ions of bay scallop hemolymph traversed in a similar direction as that of surrounding salinity.

Article Title: Estrogen Receptor-α Suppresses Liver Carcinogenesis and Establishes Sex-Specific Gene Expression.
Article Snippet: Oligo d(T)15 primers (Promega, Madison, WI, USA) and RNasin (Promega), were used in the reaction in a final volume of 20 uL.

Article Title: Toxicity response to benzo[α]pyrene exposure: Modulation of immune parameters of the bay scallop, Argopectenirradians.
Article Snippet: Bay scallops were exposed to four BaP concentrations (0.5, 1.0, 10 and 50 μg/L) for 72 h to elucidate their immune response.. Immune parameters were evaluated by measuring nitric oxide (NO) levels in hemolymph.. Additionally, we measured peptidoglycan recognition proteins (PGRP), fibrinogen-domain-containing protein (FReDC1), metallothionein (MT), and heat shock protein (HSP) 70 mRNA expression in digestive diverticula.

Article Title: Characterization and genetic diversity of MHC class II DRB genes in the Arabian camel ( Camelus dromedarius )
Article Snippet: Reverse transcription was performed using the MMLV reverse transcriptase (Invitrogen) following manufacturer protocol in a final volume of 20 μl containing 1 μg of RNA and 2.5 μM of the oligo d(T)15 primer (Promega).

Article Title: Estrogen Receptor-α Suppresses Liver Carcinogenesis and Establishes Sex-Specific Gene Expression
Article Snippet: Oligo d(T) 15 primers (Promega, Madison, WI, USA) and RNasin (Promega), were used in the reaction in a final volume of 20 uL.

Article Title: Human monoclonal antibodies against CD25
Article Snippet: RNA Preparation: Total RNA was prepared from 5×106 cells of four (4) different human CD25 hybridoma cell lines (AB1, AB7, AB11, AB12) with Rneasy kit (Qiagen, Westburg, Leusden, Netherlands) according to the manufacturer's protocol. cDNA Preparation: Complementary DNA (cDNA) of RNA from human CD25 hybridoma cells was prepared from 3 μg total RNA with AMV Reverse Transcriptase with buffer (Roche Diagnostics GmbH, Mannheim, Germany), oligo d(T)15 (Promega, Madison, Wis., USA), dNTP (Roche Diagnostics GmbH, Mannheim, Germany) and RNAsin (Promega) according to the manufacturer's protocol (2000, version 3).

cDNA Synthesis:

Article Title: Aluminum (Al) causes a delayed suppression of nucleotide excision repair (NER) capacity in zebrafish (Danio rerio) embryos via disturbance of DNA lesion detection.
Article Snippet: Aluminum (Al) is extensively used for making cooking utensils and its presence in the aquatic environment may occur through acid mine drainage and wastewater discharge.. Al is known to induce genotoxicity in human cells, rodents, and fish.. Nucleotide excision repair (NER) eliminates helix-twisting DNA lesions such as UV-induced dipyrimidine photoproducts.

Article Title: Increased expression of CD38 on endothelial cells in SARS-CoV-2 infection in cynomolgus macaques.
Article Snippet: SARS-CoV-2 infection causes activation of endothelial cells (ECs), leading to dysmorphology and dysfunction.. To study the pathogenesis of endotheliopathy, the activation of ECs in lungs of cynomolgus macaques after SARSCoV-2 infection and changes in nicotinamide adenine dinucleotide (NAD) metabolism in ECs were investigated, with a focus on the CD38 molecule, which degrades NAD in inflammatory responses after SARS-CoV-2 infection.. Activation of ECs was seen from day 3 after SARS-CoV-2 infection in macaques, with increases of intravascular fibrin and NAD metabolism-associated enzymes including CD38.

Article Title: Effects of salinity changes on the osmoregulatory and stress responses in the bay scallop Argopecten irradians
Article Snippet: Most marine mollusks exhibit osmoregulatory mechanisms to cope with hypoor hyperosmotic stress.. In the present study, we elucidated the effects of exposure to hyposmotic and hyperosmotic stress on bay scallop Argopecten irradians.. The Na+, K+, and Cl− ions of bay scallop hemolymph traversed in a similar direction as that of surrounding salinity.

Article Title: Estrogen Receptor-α Suppresses Liver Carcinogenesis and Establishes Sex-Specific Gene Expression.
Article Snippet: Oligo d(T)15 primers (Promega, Madison, WI, USA) and RNasin (Promega), were used in the reaction in a final volume of 20 uL.

Article Title: Toxicity response to benzo[α]pyrene exposure: Modulation of immune parameters of the bay scallop, Argopectenirradians.
Article Snippet: Bay scallops were exposed to four BaP concentrations (0.5, 1.0, 10 and 50 μg/L) for 72 h to elucidate their immune response.. Immune parameters were evaluated by measuring nitric oxide (NO) levels in hemolymph.. Additionally, we measured peptidoglycan recognition proteins (PGRP), fibrinogen-domain-containing protein (FReDC1), metallothionein (MT), and heat shock protein (HSP) 70 mRNA expression in digestive diverticula.

Article Title: Characterization and genetic diversity of MHC class II DRB genes in the Arabian camel ( Camelus dromedarius )
Article Snippet: Reverse transcription was performed using the MMLV reverse transcriptase (Invitrogen) following manufacturer protocol in a final volume of 20 μl containing 1 μg of RNA and 2.5 μM of the oligo d(T)15 primer (Promega).

Article Title: Estrogen Receptor-α Suppresses Liver Carcinogenesis and Establishes Sex-Specific Gene Expression
Article Snippet: Oligo d(T) 15 primers (Promega, Madison, WI, USA) and RNasin (Promega), were used in the reaction in a final volume of 20 uL.

Article Title: Human monoclonal antibodies against CD25
Article Snippet: RNA Preparation: Total RNA was prepared from 5×106 cells of four (4) different human CD25 hybridoma cell lines (AB1, AB7, AB11, AB12) with Rneasy kit (Qiagen, Westburg, Leusden, Netherlands) according to the manufacturer's protocol. cDNA Preparation: Complementary DNA (cDNA) of RNA from human CD25 hybridoma cells was prepared from 3 μg total RNA with AMV Reverse Transcriptase with buffer (Roche Diagnostics GmbH, Mannheim, Germany), oligo d(T)15 (Promega, Madison, Wis., USA), dNTP (Roche Diagnostics GmbH, Mannheim, Germany) and RNAsin (Promega) according to the manufacturer's protocol (2000, version 3).

Virus:

Article Title: Aluminum (Al) causes a delayed suppression of nucleotide excision repair (NER) capacity in zebrafish (Danio rerio) embryos via disturbance of DNA lesion detection.
Article Snippet: Aluminum (Al) is extensively used for making cooking utensils and its presence in the aquatic environment may occur through acid mine drainage and wastewater discharge.. Al is known to induce genotoxicity in human cells, rodents, and fish.. Nucleotide excision repair (NER) eliminates helix-twisting DNA lesions such as UV-induced dipyrimidine photoproducts.

Article Title: Increased expression of CD38 on endothelial cells in SARS-CoV-2 infection in cynomolgus macaques.
Article Snippet: SARS-CoV-2 infection causes activation of endothelial cells (ECs), leading to dysmorphology and dysfunction.. To study the pathogenesis of endotheliopathy, the activation of ECs in lungs of cynomolgus macaques after SARSCoV-2 infection and changes in nicotinamide adenine dinucleotide (NAD) metabolism in ECs were investigated, with a focus on the CD38 molecule, which degrades NAD in inflammatory responses after SARS-CoV-2 infection.. Activation of ECs was seen from day 3 after SARS-CoV-2 infection in macaques, with increases of intravascular fibrin and NAD metabolism-associated enzymes including CD38.

Article Title: Effects of salinity changes on the osmoregulatory and stress responses in the bay scallop Argopecten irradians
Article Snippet: Most marine mollusks exhibit osmoregulatory mechanisms to cope with hypoor hyperosmotic stress.. In the present study, we elucidated the effects of exposure to hyposmotic and hyperosmotic stress on bay scallop Argopecten irradians.. The Na+, K+, and Cl− ions of bay scallop hemolymph traversed in a similar direction as that of surrounding salinity.

Article Title: Estrogen Receptor-α Suppresses Liver Carcinogenesis and Establishes Sex-Specific Gene Expression.
Article Snippet: Oligo d(T)15 primers (Promega, Madison, WI, USA) and RNasin (Promega), were used in the reaction in a final volume of 20 uL.

Article Title: Toxicity response to benzo[α]pyrene exposure: Modulation of immune parameters of the bay scallop, Argopectenirradians.
Article Snippet: Bay scallops were exposed to four BaP concentrations (0.5, 1.0, 10 and 50 μg/L) for 72 h to elucidate their immune response.. Immune parameters were evaluated by measuring nitric oxide (NO) levels in hemolymph.. Additionally, we measured peptidoglycan recognition proteins (PGRP), fibrinogen-domain-containing protein (FReDC1), metallothionein (MT), and heat shock protein (HSP) 70 mRNA expression in digestive diverticula.

Article Title: Characterization and genetic diversity of MHC class II DRB genes in the Arabian camel ( Camelus dromedarius )
Article Snippet: Reverse transcription was performed using the MMLV reverse transcriptase (Invitrogen) following manufacturer protocol in a final volume of 20 μl containing 1 μg of RNA and 2.5 μM of the oligo d(T)15 primer (Promega).

Article Title: Estrogen Receptor-α Suppresses Liver Carcinogenesis and Establishes Sex-Specific Gene Expression
Article Snippet: Oligo d(T) 15 primers (Promega, Madison, WI, USA) and RNasin (Promega), were used in the reaction in a final volume of 20 uL.

Article Title: Human monoclonal antibodies against CD25
Article Snippet: RNA Preparation: Total RNA was prepared from 5×106 cells of four (4) different human CD25 hybridoma cell lines (AB1, AB7, AB11, AB12) with Rneasy kit (Qiagen, Westburg, Leusden, Netherlands) according to the manufacturer's protocol. cDNA Preparation: Complementary DNA (cDNA) of RNA from human CD25 hybridoma cells was prepared from 3 μg total RNA with AMV Reverse Transcriptase with buffer (Roche Diagnostics GmbH, Mannheim, Germany), oligo d(T)15 (Promega, Madison, Wis., USA), dNTP (Roche Diagnostics GmbH, Mannheim, Germany) and RNAsin (Promega) according to the manufacturer's protocol (2000, version 3).



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